Hydrogen peroxide (H₂O₂) is a product of alcohol detoxification.

• H₂O₂ is produced in peroxisomes found in all eukaryotic cells, including liver cells, where the detoxification takes place.

• Converted to water by Hydrogen peroxidase: 2H₂O₂ -> 2H₂O + O₂; the O₂ can be collected and measured in this lab.

Enzymes act by lowering the activation energy of chemical reactions, and have optimal temperature and pH conditions.

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1. TESTING THE EFFECT OF TEMPERATURE ON ENZYME ACTIVITY p. 46
   • Cut strips of filter paper 5 cm long to absorb enzyme.

   • Tray setup.

   • Record the 3 actual temperatures in the columns. 10°C=50°F; 37°C=98.6°F; 40°=104°F.
   • When entering data, the volume should increase as time intervals increase, until it levels off.

   • Graph the results. Try to put all 3 treatments for each section on one graph, so each graph should be 3 line graphs.

   • Which is the independent variable (X axis)?

2. TESTING THE EFFECT OF ENZYME CONCENTRATION ON ENZYME ACTIVITY

   Use 1/2 strip, 1 strip, 2 strips.

3. TESTING THE EFFECT OF PH ON ENZYME ACTIVITY

   Use 50% HCL and 50% NaOH, measure pH with pH paper.