Diffusion review.
Observe diffusion of KMnO₄ at 3 temperatures.
What is your hypothesis about the effect of temperature on rate of diffusion?

Record changes at 10 minute intervals for 30 - 60 minutes.

Dialysis is the separation of solutes by means of their unequal diffusion through semipermeable membranes.

1. Tie one dialysis tube; make sure it does not ;eak.
2. Mix into the tube a stock solution of 5 ml each of 3 solutes:
   • starch,
   • glucose,
   • NaCl.
     Be careful not to spill any of the solutes into the beaker.
3. After about 1 hour, apply tests:
   • Iodine (I₂KI) for starch,
   • Benedict's solution (in warm water bath) for glucose,
   • Silver nitrate (AgNO₃) for NaCl.

Osmosis review

• Hypertonic, isotonic, hypotonic environments:
  Animal cells.
  Plant cells.

• Use sucrose in 1M and 2M concentrations (1M = 6.02 * 10²³ molecules per liter).
• Measure height of water in pipet in 10-minute intervals.
  
• Hypothesis: will the water level rise or fall?

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